Optimized Fast-FISH with <FONT FACE=Symbol>a</font>-satellite probes: acceleration by microwave activation

Optimized Fast-FISH with α-satellite probes: acceleration by microwave activation

It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization) technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation) time and the number of subsequent washing steps were reduced considerably by omitting denaturing chemical agents (e.g., formamide). The appropriate hybridizatio...

Oligonucleotide probes for alpha satellite DNA variants can distinguish homologous chromosomes by FISH.

Chromosomal heteromorphisms have been used extensively to mark individual chromosomes. However, classical banding techniques used to identify these structural variants are imprecise and difficult to quantify. Different chromosomes 17 from the human population are characterized by distinct haplotypes of alpha satellite DNA. We have used these sequence variants to construct oligonuoleotide probes...

Comments on plasma diagnostics with microwave probes

Fast dynamic parallel phase contrast MRI with high acceleration factors and optimized SNR

Introduction: Time-resolved phase contrast (PC) MRI is important for many clinical applications and requires fast data acquisition. In order to increase spatiotemporal resolution or reduce total acquisition times, parallel imaging techniques such as kt-SENSE, kt-BLAST [1] and kt-Grappa [2] have been introduced. For kt-BLAST/ kt-SENSE it has been shown that high reduction factors of the order of...

Optimization of fast-fluorescence in situ hybridization with repetitive alpha-satellite probes.

A rapid FISH (fluorescence in situ hybridization) technique (Fast-FISH) for quantitative microscopy has been recently introduced. For highly repetitive DNA probes the hybridization (renaturation) time and the number of necessary washing steps were reduced considerably by omitting formamide or equivalent denaturing chemical agents. Due to low stringency conditions major and minor binding sites o...